OSA Student Chapter Alumni Talk: "Optimizing Super-Resolution Microscopy for High-Throughput Imaging and Cell Biology" by Kyle Douglass
Monday, January 9, 2017 12:00 PM to 1:00 PM
CREOL Room 103
CREOL Room 103
Abstract:
The shift in cell biology from an observational science to a more rigorous and quantitative approach was—and continues to be—driven by the development of technologies that can probe the molecular foundations of life. Super-resolution fluorescence microscopy is one such technology, enabling scientists to visualize structures inside cells with a high degree of specificity and resolutions approaching nanometer length scales. Far from being of limited applicability, super-resolution is now used in many areas of cell biology, from membrane dynamics to single molecule tracking.
Despite its continued adoption by biologists, there remain however many problems that super-resolution is currently ill-suited to address, such as resolving the structure of very dense and heterogeneous protein complexes or accounting for cell-to-cell variability. Overcoming these problems requires biologists, computer scientists, and physicists to work together and to leverage each others' expertise. In this talk, I will discuss how I am using my education in optics and working directly with biologists to build an automated, high-throughput, super-resolution system capable of imaging hundreds of cells with nanometer-scale resolutions in a matter of hours. This system solves the problems described above by more than quadrupling the imaged area over that of similar microscopes and ensuring that the resolution is uniform across the field of view. By combining the resulting big data sets with computational modeling, we are building an understanding of how proteins are packaged together inside an organelle called the centriole and how DNA is folded inside the nucleus.
Despite its continued adoption by biologists, there remain however many problems that super-resolution is currently ill-suited to address, such as resolving the structure of very dense and heterogeneous protein complexes or accounting for cell-to-cell variability. Overcoming these problems requires biologists, computer scientists, and physicists to work together and to leverage each others' expertise. In this talk, I will discuss how I am using my education in optics and working directly with biologists to build an automated, high-throughput, super-resolution system capable of imaging hundreds of cells with nanometer-scale resolutions in a matter of hours. This system solves the problems described above by more than quadrupling the imaged area over that of similar microscopes and ensuring that the resolution is uniform across the field of view. By combining the resulting big data sets with computational modeling, we are building an understanding of how proteins are packaged together inside an organelle called the centriole and how DNA is folded inside the nucleus.
Biography:
Kyle Douglass began his studies at the Rose-Hulman Institute of Technology in Terre Haute, Indiana, achieving the bachelor of science degree in engineering physics in May, 2007. He began his PhD studies at CREOL immediately afterwards, joining the lab of Professor Aristide Dogariu. While at CREOL, he worked on a number of projects related to optical sensing and control of random media, such as helping to develop a low-coherence, fiber-optics based interferometer for probing microscope relaxation times in blood and other biopolymers.
Following the completion of his PhD in August, 2013, he moved to the Laboratory of Experimental Biophysics at the École Polytechnique Fédérale de Lausanne (EPFL), which is led by Professor Suliana Manley. He is currently working directly with biologists and chemists to develop high-throughput microscopy systems for studying the structural biology of the centriole and bacterial shape dynamics. His development of a large field of view, automated STORM microscope was recently published and is now enabling his lab and collaborators to obtain unprecedented views of cell-to-cell variability at nanometer length scales.
Following the completion of his PhD in August, 2013, he moved to the Laboratory of Experimental Biophysics at the École Polytechnique Fédérale de Lausanne (EPFL), which is led by Professor Suliana Manley. He is currently working directly with biologists and chemists to develop high-throughput microscopy systems for studying the structural biology of the centriole and bacterial shape dynamics. His development of a large field of view, automated STORM microscope was recently published and is now enabling his lab and collaborators to obtain unprecedented views of cell-to-cell variability at nanometer length scales.
For Additional information:
Midya Parto
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