Seminar: “Single-molecule studies of signaling events in crude cell extracts” by Vasudha Aggarwal
Friday, October 21, 2016 3:00 PM to 4:00 PM
CREOL Room 103
CREOL Room 103
Vasudha Aggarwal
Johns Hopkins School of Medicine
Johns Hopkins School of Medicine
Abstract:
Macromolecular complexes consisting of proteins and lipids are ubiquitous during signaling. For example, phospholipid targeting by proteins is critical for the initiation of signaling cascade and protein-protein interactions are needed for signal transduction. As a result, signaling proteins are involved in heterogeneous composition, stoichiometry, order of assembly, and conformations that can change dynamically, making single-molecule studies best suited to measure these properties accurately. I will talk about single-molecule pull-down technique (SiMPull) developed in the lab of Prof. Taekjip Ha, which utilizes principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native proteins in crude cell extracts. I will show how this technique can be used to obtain mechanistic information for native lipid-protein and protein-protein complexes.
Macromolecular complexes consisting of proteins and lipids are ubiquitous during signaling. For example, phospholipid targeting by proteins is critical for the initiation of signaling cascade and protein-protein interactions are needed for signal transduction. As a result, signaling proteins are involved in heterogeneous composition, stoichiometry, order of assembly, and conformations that can change dynamically, making single-molecule studies best suited to measure these properties accurately. I will talk about single-molecule pull-down technique (SiMPull) developed in the lab of Prof. Taekjip Ha, which utilizes principles of conventional co-immunoprecipitation assay with single-molecule fluorescence microscopy to probe native proteins in crude cell extracts. I will show how this technique can be used to obtain mechanistic information for native lipid-protein and protein-protein complexes.
Biography:
Vasudha Aggarwal is pursing her PhD in Biophysics & Biophysical Chemistry in the laboratory of Prof. Taekjip Ha at Johns Hopkins School of Medicine. She is interested in single-molecule fluorescence studies of native macromolecular complexes with applications in various biological processes. She has studied protein complexes involved in DNA replication, heterochromatin formation, signaling, cell division, and membrane receptors. She obtained her MSc in Biology from Tata Institute of Fundamental Research in Mumbai, India, where she studied protein unfolding-folding properties using Atomic Force Microscope based force spectroscopy.
Vasudha Aggarwal is pursing her PhD in Biophysics & Biophysical Chemistry in the laboratory of Prof. Taekjip Ha at Johns Hopkins School of Medicine. She is interested in single-molecule fluorescence studies of native macromolecular complexes with applications in various biological processes. She has studied protein complexes involved in DNA replication, heterochromatin formation, signaling, cell division, and membrane receptors. She obtained her MSc in Biology from Tata Institute of Fundamental Research in Mumbai, India, where she studied protein unfolding-folding properties using Atomic Force Microscope based force spectroscopy.
For more information:
Kyu Young Han
Kyu Young Han
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